![]() ![]() On the one hand, the positive effect of green synthesized Ag-NPs on cancer cells and the induction of apoptosis by them has been proven 10, and on the other hand, the biocompatibility, low toxicity, safety and drug-carrying capacity of HNTs nanocarriers have been confirmed for the body 11, since we decided, for the first time, to embellish and load of HNTs with Ag-NPs to increase the solubility and check their function on Jurkat floating cells. proved that changing the size and morphology of Ag-NPs can significantly affect their long-term stability in biological environments since small NPs release more Ag + than large particles and are therefore more soluble 9. Also, by disrupting the surface charge responsible for electrostatic repulsion between NPs, the possibility of particle accumulation in biological fluids increases 8. In general, parameters affecting the dissolution of Ag-NPs include surface coating, temperature, size, morphology, ionic strength, and pH 7. Silver nanoparticles (Ag-NPs) may not have good solubility in water due to low surface charge or a size larger than 50 nm, and it is necessary to take measures for their solubility. On the other hand, due to the high lumen volume, high length-to-diameter ratio, and better solubility, HNTs can be used as an ideal natural nano-carrier to increase the solubility of insoluble or poorly soluble compounds in water 3. So, depending on the molecular mass, the interactions between drugs and HNTs, and the solubility of drugs, drug release from HNTs may take several hours to fifteen days 6. Therefore, HNTs that possess large surface areas can capture drugs through adsorption to the outer walls or inside the tubules, or load drugs into the lumen or even between the layers 5. In the structure of this natural nanomaterial, the Al–OH groups are folded inside and the Si–O–Si groups are exposed to the outer surface so that at neutral pH, their inner surface has a positive charge and their outer surface has a negative charge 3, 4. The hollow tubular structure of HNT with a lumen of 10–40 nm, an outer diameter of 50–70 nm, and a length of about 100–1000 nm has generated a growing research interest aimed at developing new organic or inorganic nanocomposites 2. ![]() Halloysite nanotubes (HNTs) are inorganic natural aluminosilicates that can be used as biocompatible and sustainable excipients in the medical and chemical industries 1. Therefore, on the one hand, we can hope for the ability of HNTs-Ag NPs to induce apoptosis in blood cancer cells and on the other hand for its protective effects on normal blood cells. Finally, the reduction of malondialdehyde production after 24 h proved that the presence of HNTs can have a good protective effect on lipid peroxidation in erythrocytes. Also, the expression of the caspase-3 gene has increased 10 times compared to the control. ![]() Investigating the expression of genes involved in apoptosis by Real-time PCR proved that decorated HNTs with Ag NPs can increase the Bak1/Bclx ratio by 17.5 times the control group. The IC 50 value was estimated as 0.00177 mg/mL after 96 h of treatment. Cell survival assay by the MTT method showed that HNTs-Ag NPs can decrease the survival of Jurkat T-cells to about 10% compared to the control. Therefore, for the first time, we decorated halloysite nanotubes (HNTs) with green silver nanoparticles (Ag NPs) with the help of Moringa Peregrina leaves extract to increase the solubility of Ag NPs and to use the protective ability of HNTs against lipid peroxidation in erythrocytes. Since leukemia chemotherapy usually fails due to chemotherapy resistance and disease relapse, many efforts are being made to develop new methods of leukemia treatment. Leukemia is the 15th most common cancer in adults and the first most common cancer in children under the age of five, and unfortunately, it accounts for many deaths every year. ![]()
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